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Moderate changes of the K m app values in combination with the altered k cat app values resulted in decreased apparent catalytic efficiencies of E170A/K for both substrates as compared with the wild-type enzyme and E170D (Fig

Statistical analysis The data analyses were conducted using GraphPad Prism, version 8, developed by GraphPad Software in San Diego, CA, USA

Pulsing antibiotics means using an antibiotic, not steadily seven days a week, but using it, lets say two or three days in a row and then stopping and having no treatment for the rest of the week